Modeling BCR/ABL-driven malignancies in the mouse

Methods Mol Biol. 2015:1267:263-82. doi: 10.1007/978-1-4939-2297-0_12.

Abstract

In this chapter, we describe model systems to study leukemia driven by the Abelson oncogene. In people, the Abelson oncogene results from the chromosomal translocation t(9;22)(q34;q11) that is found in more than 90 % of all human chronic myeloid leukemia (CML) patients and in 20-25 % of patients suffering from acute lymphoid leukemia (ALL). This translocation is also called Philadelphia chromosome and encodes the BCR/ABL oncogene, a constitutive active tyrosine kinase. BCR/ABL renders hematopoietic cells independent from exogenous growth-stimulatory signals by continuously engaging signaling pathways including JAK-STAT signaling and the MAPK pathway. The enforced expression of BCR/ABL suffices to transform hematopoietic cells which made it to one of the best studied model systems in the field. Here we present methods to study BCR/ABL-triggered leukemia and solid lymphoid tumor formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Bone Marrow Transplantation
  • Cell Line, Tumor
  • Coculture Techniques
  • Disease Models, Animal*
  • Female
  • Fusion Proteins, bcr-abl / genetics*
  • Gene Deletion
  • Hematopoiesis
  • Humans
  • Leukemia, Lymphoid / genetics*
  • Leukemia, Lymphoid / pathology
  • Leukemia, Lymphoid / surgery
  • Male
  • Mice
  • Pregnancy
  • Retroviridae / genetics
  • Species Specificity

Substances

  • Fusion Proteins, bcr-abl