Insulin-like growth factor-1 endues monocytes with immune suppressive ability to inhibit inflammation in the intestine

Rong-Ti Ge; Li-Hua Mo; Ruijin Wu; Jiang-Qi Liu; Huan-Ping Zhang; Zhigang Liu; Zhanju Liu; Ping-Chang Yang

doi:10.1038/srep07735

Insulin-like growth factor-1 endues monocytes with immune suppressive ability to inhibit inflammation in the intestine

Sci Rep. 2015 Jan 15:5:7735. doi: 10.1038/srep07735.

Authors

Rong-Ti Ge¹, Li-Hua Mo², Ruijin Wu³, Jiang-Qi Liu⁴, Huan-Ping Zhang⁴, Zhigang Liu², Zhanju Liu³, Ping-Chang Yang⁵

Affiliations

¹ 1] Department of Gastroenterology, The Shanghai Tenth People's Hospital of Tongji University, Shanghai, 200072, China [2] Brain Body Institute, McMaster University, Hamilton, ON, Canada L8N 4A6.
² Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine and State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen, 518060, China.
³ Department of Gastroenterology, The Shanghai Tenth People's Hospital of Tongji University, Shanghai, 200072, China.
⁴ Brain Body Institute, McMaster University, Hamilton, ON, Canada L8N 4A6.
⁵ 1] Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine and State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen, 518060, China [2] Brain Body Institute, McMaster University, Hamilton, ON, Canada L8N 4A6.

Abstract

The pathogenesis of some chronic inflammation such as inflammatory bowel disease is unclear. Insulin-like growth factor-1 (IGF1) has active immune regulatory capability. This study aims to investigate into the mechanism by which IGF1 modulates the monocyte (Mo) properties to inhibit immune inflammation in the intestine. In this study, the production of IGF1 by intestinal epithelial cells was evaluated by real time RT-PCR and Western blotting. Mos were analyzed by flow cytometry. A mouse colitis model was created with trinitrobenzene sulfonic acid. The results showed that mouse IECs produced IGF1, which could be up regulated by exposure to CpG-ODN (CpG-oligodeoxynueleotides) in the culture. Culture the CpG-ODN-primed IEC cells and Mos or exposure of Mos to IGF1 in the culture induced the Mos to express IL-10. The IGF1-primed Mos showed the immune suppressive effect on inhibiting the immune inflammation in the mouse colon. In conclusion, the IGF1-primed Mos are capable of suppressing immune inflammation in the intestine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Cell Separation
Colitis / immunology
Colitis / pathology
Epithelial Cells / drug effects
Epithelial Cells / metabolism
Epithelial Cells / pathology
Immunosuppression Therapy*
Inflammation / immunology*
Inflammation / pathology*
Insulin-Like Growth Factor I / metabolism*
Interleukin-10 / metabolism
Intestines / immunology*
Intestines / pathology*
Lipopolysaccharide Receptors / metabolism
Mice, Inbred BALB C
Monocytes / drug effects
Monocytes / metabolism*
Oligodeoxyribonucleotides / pharmacology
Phenotype
Receptor, IGF Type 1 / metabolism
Spleen / cytology
Trinitrobenzenesulfonic Acid

Substances

CPG-oligonucleotide
Lipopolysaccharide Receptors
Oligodeoxyribonucleotides
Interleukin-10
Insulin-Like Growth Factor I
Trinitrobenzenesulfonic Acid
Receptor, IGF Type 1