Application of the Human Intestinal Tract Chip to the non-human primate gut microbiota

Benef Microbes. 2015;6(3):271-6. doi: 10.3920/BM2014.0087.

Abstract

The human intestinal microbiota is responsible for various health-related functions, and its diversity can be readily mapped with the 16S ribosomal RNA targeting Human Intestinal Tract (HIT) Chip. Here we characterise distal gut samples from chimpanzees, gorillas and marmosets, and compare them with human gut samples. Our results indicated applicability of the HITChip platform can be extended to chimpanzee and gorilla faecal samples for analysis of microbiota composition and enterotypes, but not to the evolutionary more distant marmosets.

Keywords: enterotypes; microbiota; non-human primates; phylogenetic profile.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / isolation & purification*
  • DNA, Bacterial / genetics
  • Feces / microbiology
  • Gastrointestinal Microbiome*
  • Gorilla gorilla / microbiology*
  • Humans
  • Intestines / microbiology*
  • Oligonucleotide Array Sequence Analysis
  • Pan troglodytes / microbiology*
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 16S