Β-catenin is involved in Bex2 down-regulation induced glioma cell invasion/migration inhibition

Biochem Biophys Res Commun. 2015 Jan 2;456(1):494-9. doi: 10.1016/j.bbrc.2014.11.113. Epub 2014 Dec 6.

Abstract

Previously, we found that brain expressed X-linked gene 2 (Bex2) regulates the invasion/migration ability of glioma cells. However, the mechanism of this effect remains unknown. In current study, we reported that Bex2 down-regulation inhibited glioma cell migration and invasion by decreasing the nucleus and cytoplasm protein level of β-catenin. We found that the protein levels of Bex2 and β-catenin were up-regulated and showed direct correlation in glioma tissues. Bex2 down-regulation significantly decreased β-catenin protein levels but not its mRNA levels. Furthermore, the decreased protein level of β-catenin was located in the nucleus and cytoplasm but not in the cell membrane. Further study found that the effects of Bex2 down-regulation on the invasion and migration of glioma cell could be reversed by β-catenin over-expression. Taken together, Bex2 affects the invasion and migration ability of glioma cells by regulating β-catenin.

Keywords: Bex2; Glioblastoma; Invasion; Migration; β-Catenin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Brain / metabolism
  • Brain Neoplasms / metabolism
  • Cell Line, Tumor
  • Cell Membrane / metabolism
  • Cell Movement
  • Cytoplasm / metabolism
  • Gene Expression Regulation, Neoplastic*
  • Glioma / metabolism*
  • Glioma / pathology
  • Humans
  • Neoplasm Invasiveness
  • Nerve Tissue Proteins / metabolism*
  • RNA, Small Interfering / metabolism
  • Wound Healing
  • beta Catenin / metabolism*

Substances

  • BEX2 protein, human
  • CTNNB1 protein, human
  • Nerve Tissue Proteins
  • RNA, Small Interfering
  • beta Catenin