Deletion of the ORF9p acidic cluster impairs the nuclear egress of varicella-zoster virus capsids

Laura Riva; Marc Thiry; Marielle Lebrun; Laurent L'homme; Jacques Piette; Catherine Sadzot-Delvaux

doi:10.1128/JVI.03215-14

Deletion of the ORF9p acidic cluster impairs the nuclear egress of varicella-zoster virus capsids

J Virol. 2015 Feb;89(4):2436-41. doi: 10.1128/JVI.03215-14. Epub 2014 Dec 3.

Authors

Laura Riva¹, Marc Thiry², Marielle Lebrun¹, Laurent L'homme³, Jacques Piette⁴, Catherine Sadzot-Delvaux⁵

Affiliations

¹ University of Liege, GIGA Infection Immunity and Inflammation, Laboratory of Virology and Immunology, Liege, Belgium.
² University of Liege, GIGA Neurosciences, Laboratory of Cellular and Tissular Biology, Liege, Belgium.
³ University of Liege, GIGA Signal Transduction, Laboratory of Virology and Immunology, Liege, Belgium.
⁴ University of Liege, GIGA Infection Immunity and Inflammation, Laboratory of Virology and Immunology, Liege, Belgium University of Liege, GIGA Signal Transduction, Laboratory of Virology and Immunology, Liege, Belgium.
⁵ University of Liege, GIGA Infection Immunity and Inflammation, Laboratory of Virology and Immunology, Liege, Belgium csadzot@ulg.ac.be.

Abstract

The protein encoded by ORF9 is essential for varicella-zoster virus (VZV) replication. Previous studies documented its presence in the trans-Golgi network and its involvement in secondary envelopment. In this work, we deleted the ORF9p acidic cluster, destroying its interaction with ORF47p, and this resulted in a nuclear accumulation of both proteins. This phenotype results in an accumulation of primary enveloped capsids in the perinuclear space, reflecting a capsid de-envelopment defect.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Capsid / metabolism*
Cell Nucleus / virology
Herpesvirus 3, Human / genetics
Herpesvirus 3, Human / physiology*
Sequence Deletion*
Viral Proteins / genetics*
Virus Release*
Virus Replication*

Substances

Viral Proteins