DNA methylation dynamics of a maternally methylated DMR in the mouse Dlk1-Dio3 domain

Tie-Bo Zeng; Hong-Juan He; Zheng-Bin Han; Feng-Wei Zhang; Zhi-Jun Huang; Qi Liu; Wei Cui; Qiong Wu

doi:10.1016/j.febslet.2014.10.038

DNA methylation dynamics of a maternally methylated DMR in the mouse Dlk1-Dio3 domain

FEBS Lett. 2014 Dec 20;588(24):4665-71. doi: 10.1016/j.febslet.2014.10.038. Epub 2014 Nov 11.

Authors

Tie-Bo Zeng¹, Hong-Juan He¹, Zheng-Bin Han¹, Feng-Wei Zhang¹, Zhi-Jun Huang¹, Qi Liu¹, Wei Cui¹, Qiong Wu²

Affiliations

¹ School of Life Science and Technology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China.
² School of Life Science and Technology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China. Electronic address: kigo@hit.edu.cn.

PMID: 25447521
DOI: 10.1016/j.febslet.2014.10.038

Abstract

The mouse delta-like homolog 1 and type III iodothyronine deiodinase (Dlk1-Dio3) imprinted domain contains three known paternally methylated differentially methylated regions (DMRs): intergenic DMR (IG-DMR), maternally expressed 3-DMR (Gtl2-DMR), and Dlk1-DMR. Here, we report the first maternally methylated DMR, CpG island 2 (CGI-2), is located approximately 800 bp downstream of miR-1188. CGI-2 is highly methylated in sperm and oocytes, de-methylated in pre-implantation embryos, and differentially re-methylated during post-implantation development. CGI-2, similarly to Gtl2-DMR and Dlk1-DMR, acquires differential methylation prior to embryonic day 7.5 (E7.5). Both H3K4me3 and H3K9me3 histone modifications are enriched at CGI-2. Furthermore, CCCTC-binding factor (CTCF) binds to both alleles of CGI-2 in vivo. These results contribute to the investigation of imprinting regulation in this domain.

Keywords: CCCTC-binding factor; DNA methylation; Differentially methylated region; Dlk1–Dio3 domain; Genomic imprinting.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
CCCTC-Binding Factor
Calcium-Binding Proteins
CpG Islands / genetics
DNA Methylation*
Embryo Implantation
Embryonic Development / genetics
Female
Genomic Imprinting*
Histones / chemistry
Histones / metabolism
Intercellular Signaling Peptides and Proteins / genetics*
Introns / genetics
Iodide Peroxidase / genetics*
Mice
MicroRNAs / genetics
Mothers*
Pregnancy
Repressor Proteins / metabolism

Substances

CCCTC-Binding Factor
Calcium-Binding Proteins
Ctcf protein, mouse
Dlk1 protein, mouse
Histones
Intercellular Signaling Peptides and Proteins
MicroRNAs
Repressor Proteins
iodothyronine deiodinase type III
Iodide Peroxidase