MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy

Cell Death Dis. 2014 Oct 23;5(10):e1479. doi: 10.1038/cddis.2014.430.

Abstract

Diabetic cardiomyopathy is a common cardiac condition in patients with diabetes mellitus, which can result in cardiac hypertrophy and subsequent heart failure, associated with pyroptosis, the pro-inflammatory programmed cell death. MicroRNAs (miRNAs), small endogenous non-coding RNAs, have been shown to be involved in diabetic cardiomyopathy. However, whether miRNAs regulate pyroptosis in diabetic cardiomyopathy remains unknown. Our study revealed that mir-30d expression was substantially increased in streptozotocin (STZ)-induced diabetic rats and in high-glucose-treated cardiomyocytes as well. Upregulation of mir-30d promoted cardiomyocyte pyroptosis in diabetic cardiomyopathy; conversely, knockdown of mir-30d attenuated it. In an effort to understand the signaling mechanisms underlying the pro-pyroptotic property of mir-30d, we found that forced expression of mir-30d upregulated caspase-1 and pro-inflammatory cytokines IL-1β and IL-18. Moreover, mir-30d directly repressed foxo3a expression and its downstream protein, apoptosis repressor with caspase recruitment domain (ARC). Furthermore, silencing ARC by siRNA mimicked the action of mir-30d: upregulating caspase-1 and inducing pyroptosis. These findings promoted us to propose a new signaling pathway leading to cardiomyocyte pyroptosis under hyperglycemic conditions: mir-30d↑→foxo3a↓→ ARC↓→caspase-1↑→IL-1β, IL-18↑→pyroptosis↑. Therefore, mir-30d may be a promising therapeutic target for the management of diabetic cardiomyopathy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis* / genetics
  • Base Sequence
  • Caspase 1 / metabolism
  • Cytoskeletal Proteins / metabolism
  • Diabetes Mellitus, Experimental / physiopathology
  • Diabetic Cardiomyopathies / genetics
  • Diabetic Cardiomyopathies / metabolism*
  • Diabetic Cardiomyopathies / pathology*
  • Diabetic Cardiomyopathies / physiopathology
  • Down-Regulation / drug effects
  • Down-Regulation / genetics
  • Forkhead Box Protein O3
  • Forkhead Transcription Factors / genetics
  • Forkhead Transcription Factors / metabolism*
  • Glucose / toxicity
  • Heart / drug effects
  • Heart / physiopathology
  • Hyperglycemia / genetics
  • Hyperglycemia / pathology
  • Inflammation / genetics
  • Inflammation / pathology*
  • MicroRNAs / metabolism*
  • Models, Biological
  • Molecular Sequence Data
  • Myocytes, Cardiac / drug effects
  • Myocytes, Cardiac / metabolism
  • Myocytes, Cardiac / pathology*
  • Myocytes, Cardiac / ultrastructure
  • Nerve Tissue Proteins / metabolism
  • Rats
  • Rats, Wistar
  • Streptozocin
  • Up-Regulation / drug effects
  • Up-Regulation / genetics

Substances

  • Cytoskeletal Proteins
  • FOXO3 protein, rat
  • Forkhead Box Protein O3
  • Forkhead Transcription Factors
  • MIRN30 microRNA, rat
  • MicroRNAs
  • Nerve Tissue Proteins
  • activity regulated cytoskeletal-associated protein
  • Streptozocin
  • Caspase 1
  • Glucose