The ambiguous boundary between EBV-related hemophagocytic lymphohistiocytosis and systemic EBV-driven T cell lymphoproliferative disorder

Int J Clin Exp Pathol. 2014 Aug 15;7(9):5738-49. eCollection 2014.

Abstract

Epstein Barr virus (EBV)-related hemophagocytic lymphohistiocytosis (EBV-HLH) is a form of acquired, infection-related HLH which typically represents a fulminant presentation of an acute EBV infection of CD8+ T cells with 30-50% mortality rate. Systemic EBV-positive lymphoproliferative disease of childhood (SE-LPD) is a rare T cell lymphoproliferative disorder predominantly arising in the setting of acute EBV infection, often presenting with HLH. Since both entities have been associated with clonal T cell populations, the discrimination between these diseases is often ambiguous. We report a unique case of a 21 years old female who presented with clinical and laboratory findings of florid HLH in the setting of markedly elevated EBV titers (>1 million) and an aberrant T cell population shown to be clonal by flow cytometry, karyotype, and molecular studies. This case raises the differential of EBV-HLH versus SE-LPD. Review of the literature identified 74 cases of reported EBV-HLH and 21 cases of SE-LPD with associated HLH in 25 studies. Of those cases with available outcome data, 62 of 92 cases (67%) were fatal. Of 60 cases in which molecular clonality was demonstrated, 37 (62%) were fatal, while all 14 cases (100%) demonstrating karyotypic abnormalities were fatal. Given the karyotypic findings in this sentinel case, a diagnosis of SE-LPD was rendered. The overlapping clinical and pathologic findings suggest that EBV-HLH and SE-LPD are a biologic continuum, rather than discrete entities. The most clinically useful marker of mortality was an abnormal karyotype rather than other standards of clonality assessment.

Keywords: EBV-related HLH; EBV-related T cell lymphoma; Systemic EBV-positive lymphoproliferative disease of childhood; atypical T cell population; clonal EBV-related HLH.

Publication types

  • Case Reports
  • Review

MeSH terms

  • Biopsy
  • Diagnosis, Differential
  • Epstein-Barr Virus Infections / diagnosis*
  • Epstein-Barr Virus Infections / genetics
  • Epstein-Barr Virus Infections / immunology
  • Epstein-Barr Virus Infections / pathology
  • Epstein-Barr Virus Infections / virology
  • Female
  • Flow Cytometry
  • Herpesvirus 4, Human / isolation & purification
  • Humans
  • Immunohistochemistry
  • Karyotyping
  • Lymphohistiocytosis, Hemophagocytic / diagnosis*
  • Lymphohistiocytosis, Hemophagocytic / genetics
  • Lymphohistiocytosis, Hemophagocytic / immunology
  • Lymphohistiocytosis, Hemophagocytic / pathology
  • Lymphohistiocytosis, Hemophagocytic / virology
  • Lymphoma, T-Cell / diagnosis*
  • Lymphoma, T-Cell / genetics
  • Lymphoma, T-Cell / immunology
  • Lymphoma, T-Cell / pathology
  • Lymphoma, T-Cell / surgery
  • Lymphoma, T-Cell / virology
  • Peripheral Blood Stem Cell Transplantation
  • Predictive Value of Tests
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / virology
  • Treatment Outcome
  • Young Adult