Derivation of GMP-compliant integration-free hiPSCs using modified mRNAs

Methods Mol Biol. 2015:1283:31-42. doi: 10.1007/7651_2014_124.

Abstract

The clinical use of human induced pluripotent stem cells (hiPSCs) and the development of patients-specific gene and cell therapies rely on the development of fast, reliable, and integration-free methods of derivation of pluripotent stem cells from somatic tissues. Here we describe an integration-free protocol for the rapid derivation of hiPSCs from dermal fibroblasts using modified mRNAs. This method is inexpensive, highly efficient, and makes use of reagents that are xeno-free and chemically defined and can therefore be adopted by any Good Manufacturing Practice (GMP) facility.

MeSH terms

  • Cell Culture Techniques / methods*
  • Cell Culture Techniques / standards*
  • Cell Differentiation / genetics*
  • Cell- and Tissue-Based Therapy / methods
  • Cell- and Tissue-Based Therapy / standards
  • Cellular Reprogramming / genetics*
  • Cryopreservation / methods
  • Cryopreservation / standards
  • Fibroblasts / cytology
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism*
  • RNA, Messenger / genetics*
  • Transfection / methods

Substances

  • RNA, Messenger