A 3D cellular context for the macromolecular world

Ardan Patwardhan; Alun Ashton; Robert Brandt; Sarah Butcher; Raffaella Carzaniga; Wah Chiu; Lucy Collinson; Pascal Doux; Elizabeth Duke; Mark H Ellisman; Erik Franken; Kay Grünewald; Jean-Karim Heriche; Abraham Koster; Werner Kühlbrandt; Ingvar Lagerstedt; Carolyn Larabell; Catherine L Lawson; Helen R Saibil; Eduardo Sanz-García; Sriram Subramaniam; Paul Verkade; Jason R Swedlow; Gerard J Kleywegt

doi:10.1038/nsmb.2897

A 3D cellular context for the macromolecular world

Nat Struct Mol Biol. 2014 Oct;21(10):841-5. doi: 10.1038/nsmb.2897.

Authors

Ardan Patwardhan¹, Alun Ashton², Robert Brandt³, Sarah Butcher⁴, Raffaella Carzaniga⁵, Wah Chiu⁶, Lucy Collinson⁵, Pascal Doux³, Elizabeth Duke², Mark H Ellisman⁷, Erik Franken⁸, Kay Grünewald⁹, Jean-Karim Heriche¹⁰, Abraham Koster¹¹, Werner Kühlbrandt¹², Ingvar Lagerstedt¹, Carolyn Larabell¹³, Catherine L Lawson¹⁴, Helen R Saibil¹⁵, Eduardo Sanz-García¹, Sriram Subramaniam¹⁶, Paul Verkade¹⁷, Jason R Swedlow¹⁸, Gerard J Kleywegt¹

Affiliations

¹ Protein Data Bank in Europe, European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, UK.
² Diamond Light Source, Didcot, UK.
³ FEI Visualization Sciences Group, Mérignac, France.
⁴ Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
⁵ Electron Microscopy Unit, Cancer Research UK London Research Institute, London, UK.
⁶ National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas.
⁷ Center for Research in Biological Systems, National Center for Microscopy and Imaging Research (NCMIR), University of California, San Diego, San Diego, California, USA.
⁸ FEI Electron Optics B.V., Eindhoven, the Netherlands.
⁹ Division of Structural Biology, Wellcome Trust Centre for Human Genetics, Oxford, UK.
¹⁰ Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
¹¹ Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, the Netherlands.
¹² Department of Structural Biology, Max Planck Institute for Biophysics, Frankfurt, Germany.
¹³ Department of Anatomy, University of California, San Francisco, San Francisco, California, USA.
¹⁴ Research Collaboratory for Structural Bioinformatics, Rutgers University, Piscataway, New Jersey, USA.
¹⁵ Institute of Structural and Molecular Biology, Department of Crystallography, Birkbeck College, London, UK.
¹⁶ Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA.
¹⁷ Wolfson Bioimaging Facility, School of Biochemistry, University of Bristol, Bristol, UK.
¹⁸ Centre for Gene Regulation and Expression, University of Dundee, Dundee, UK.

Abstract

We report the outcomes of the discussion initiated at the workshop entitled A 3D Cellular Context for the Macromolecular World and propose how data from emerging three-dimensional (3D) cellular imaging techniques—such as electron tomography, 3D scanning electron microscopy and soft X-ray tomography—should be archived, curated, validated and disseminated, to enable their interpretation and reuse by the biomedical community.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Cells / ultrastructure*
Data Curation / methods*
Electron Microscope Tomography*
Humans
Imaging, Three-Dimensional*
Macromolecular Substances / ultrastructure*
Microscopy, Electron, Scanning
Molecular Conformation
Tomography, X-Ray*

Substances

Macromolecular Substances

Abstract

Publication types

MeSH terms

Substances

Grants and funding