Effects of systemic administration of kynurenic acid and glycine on renal haemodynamics and excretion in normotensive and spontaneously hypertensive rats

Abstract

Both NMDA receptor and kynurenic acid (KYNA), a glycine-site NMDA receptor antagonist, are present in the kidney yet their functional role remains unclear. Our aim was to examine effects of intravenous KYNA and glycine on arterial blood pressure (MAP) and renal haemodynamics and excretion in anaesthetized normotensive Sprague-Dawley (S-D) and in spontaneously hypertensive (SHR) rats. Renal blood flow (RBF, renal artery probe) and renal cortical (CBF) and outer- and inner medullary perfusion (laser-Doppler) were measured, along with diuresis (V) and sodium excretion (UNaV). KYNA given alone (150mgkg(-1) iv) or during infusion of glycine at 1gkg(-1)h(-1) iv (G+K) increased or decreased RBF, respectively, in both S-D and SHR. Neither treatment altered MAP. In both strains glycine alone increased RBF and CBF 50-60% and was clearly diuretic and natriuretic, less so in SHR. KYNA increased UNaV by 4.1±1.7μmolmin(-1)and V by 11.1±4.3μlmin(-1) in S-D (P<0.05 for both); the respective increases in SHR were by 1.7±0.6μmolmin(-1) and 4.7±1.7μlmin(-1) (P<0.02 for both). G+K treatment increased UNaV by 5.2±1.4μmolmin(-1) (P<0.01) and V by 29.6±4.6μmolmin(-1) (P<0.001) in S-D, and by 2.7±0.7μmolmin(-1) (P<0.05) and 19.3±3.5μlmin(-1) (P<0.0006) in SHR. In conclusion, KYNA increased renal excretion, apparently by inhibiting tubular reabsorption, whereas glycine substantially increased renal haemodynamics by an ill-defined mechanism, with a secondary increase in the excretion. Combined G+K treatment could be utilised to combat body fluid retention and possibly alleviate hypertension, without endangering renal perfusion and function.

Keywords: Glycine; Kynurenic acid; Renal excretion; Renal haemodynamics.