FOXO1 repression contributes to block of plasma cell differentiation in classical Hodgkin lymphoma

Blood. 2014 Nov 13;124(20):3118-29. doi: 10.1182/blood-2014-07-590570. Epub 2014 Sep 17.

Abstract

The survival of classical Hodgkin lymphoma (cHL) cells depends on activation of NF-κB, JAK/STAT, and IRF4. Whereas these factors typically induce the master regulator of plasma cell (PC) differentiation PRDM1/BLIMP-1, levels of PRDM1 remain low in cHL. FOXO1, playing a critical role in normal B-cell development, acts as a tumor suppressor in cHL, but has never been associated with induction of PC differentiation. Here we show that FOXO1 directly upregulates the full-length isoform PRDM1α in cHL cell lines. We also observed a positive correlation between FOXO1 and PRDM1 expression levels in primary Hodgkin-Reed-Sternberg cells. Further, we show that PRDM1α acts as a tumor suppressor in cHL at least partially by blocking MYC. Here we provide a link between FOXO1 repression and PRDM1α downregulation in cHL and identify PRDM1α as a tumor suppressor in cHL. The data support a potential role for FOXO transcription factors in normal PC differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Cell Line, Tumor
  • Down-Regulation
  • Forkhead Box Protein O1
  • Forkhead Transcription Factors / genetics
  • Forkhead Transcription Factors / metabolism*
  • Gene Expression Regulation, Neoplastic*
  • Hodgkin Disease / genetics*
  • Hodgkin Disease / metabolism
  • Hodgkin Disease / pathology*
  • Humans
  • Plasma Cells / cytology
  • Plasma Cells / metabolism
  • Plasma Cells / pathology*
  • Positive Regulatory Domain I-Binding Factor 1
  • Proto-Oncogene Proteins c-myc / metabolism
  • Reed-Sternberg Cells / metabolism
  • Reed-Sternberg Cells / pathology
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Tumor Cells, Cultured
  • Up-Regulation

Substances

  • FOXO1 protein, human
  • Forkhead Box Protein O1
  • Forkhead Transcription Factors
  • Proto-Oncogene Proteins c-myc
  • Repressor Proteins
  • PRDM1 protein, human
  • Positive Regulatory Domain I-Binding Factor 1

Associated data

  • GEO/GSE29545