Binning metagenomic contigs by coverage and composition

Nat Methods. 2014 Nov;11(11):1144-6. doi: 10.1038/nmeth.3103. Epub 2014 Sep 14.

Abstract

Shotgun sequencing enables the reconstruction of genomes from complex microbial communities, but because assembly does not reconstruct entire genomes, it is necessary to bin genome fragments. Here we present CONCOCT, a new algorithm that combines sequence composition and coverage across multiple samples, to automatically cluster contigs into genomes. We demonstrate high recall and precision on artificial as well as real human gut metagenome data sets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Bifidobacterium / genetics
  • Contig Mapping / methods*
  • Escherichia coli K12 / genetics
  • Feces / microbiology
  • Gastrointestinal Tract / microbiology*
  • Genome, Bacterial / genetics*
  • Humans
  • Metagenome / genetics*
  • Metagenomics / methods*
  • Microbiota / genetics*
  • Sequence Analysis, DNA / methods*
  • Shiga-Toxigenic Escherichia coli / genetics
  • Software*