HLA-DQA1-HLA-DRB1 variants confer susceptibility to pancreatitis induced by thiopurine immunosuppressants

Nat Genet. 2014 Oct;46(10):1131-4. doi: 10.1038/ng.3093. Epub 2014 Sep 14.

Abstract

Pancreatitis occurs in approximately 4% of patients treated with the thiopurines azathioprine or mercaptopurine. Its development is unpredictable and almost always leads to drug withdrawal. We identified patients with inflammatory bowel disease (IBD) who had developed pancreatitis within 3 months of starting these drugs from 168 sites around the world. After detailed case adjudication, we performed a genome-wide association study on 172 cases and 2,035 controls with IBD. We identified strong evidence of association within the class II HLA region, with the most significant association identified at rs2647087 (odds ratio 2.59, 95% confidence interval 2.07-3.26, P = 2 × 10(-16)). We replicated these findings in an independent set of 78 cases and 472 controls with IBD matched for drug exposure. Fine mapping of the HLA region identified association with the HLA-DQA1*02:01-HLA-DRB1*07:01 haplotype. Patients heterozygous at rs2647087 have a 9% risk of developing pancreatitis after administration of a thiopurine, whereas homozygotes have a 17% risk.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azathioprine / adverse effects
  • Azathioprine / chemistry
  • Azathioprine / metabolism
  • Gene Frequency
  • Genetic Predisposition to Disease / genetics*
  • Genome-Wide Association Study
  • Genotype
  • HLA-DQ alpha-Chains / chemistry
  • HLA-DQ alpha-Chains / genetics*
  • HLA-DQ alpha-Chains / metabolism
  • HLA-DRB1 Chains / chemistry
  • HLA-DRB1 Chains / genetics*
  • HLA-DRB1 Chains / metabolism
  • Haplotypes
  • Humans
  • Immunosuppressive Agents / adverse effects
  • Immunosuppressive Agents / chemistry
  • Immunosuppressive Agents / metabolism
  • Inflammatory Bowel Diseases / drug therapy
  • Mercaptopurine / adverse effects
  • Mercaptopurine / chemistry
  • Mercaptopurine / metabolism
  • Models, Molecular
  • Molecular Structure
  • Pancreatitis / chemically induced
  • Pancreatitis / genetics*
  • Polymorphism, Single Nucleotide*
  • Protein Binding
  • Protein Structure, Tertiary
  • Risk Factors

Substances

  • HLA-DQ alpha-Chains
  • HLA-DQA1 antigen
  • HLA-DRB1 Chains
  • Immunosuppressive Agents
  • Mercaptopurine
  • Azathioprine