Broadly neutralizing antibodies suppress HIV in the persistent viral reservoir

Proc Natl Acad Sci U S A. 2014 Sep 9;111(36):13151-6. doi: 10.1073/pnas.1414148111. Epub 2014 Aug 25.

Abstract

Several highly potent and broadly neutralizing monoclonal antibodies against HIV have recently been isolated from B cells of infected individuals. However, the effects of these antibodies on the persistent viral reservoirs in HIV-infected individuals receiving antiretroviral therapy (ART) are unknown. We show that several HIV-specific monoclonal antibodies--in particular, PGT121, VRC01, and VRC03--potently inhibited entry into CD4(+) T cells of HIV isolated from the latent viral reservoir of infected individuals whose plasma viremia was well controlled by ART. In addition, we demonstrate that HIV replication in autologous CD4(+) T cells derived from infected individuals receiving ART was profoundly suppressed by three aforementioned and other HIV-specific monoclonal antibodies. These findings have implications for passive immunotherapy as an approach toward controlling plasma viral rebound in patients whose ART is withdrawn.

Keywords: HIV envelope protein; HIV-specific antibodies; latent HIV.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antibodies, Neutralizing / immunology*
  • Antiretroviral Therapy, Highly Active
  • CD4-Positive T-Lymphocytes / immunology
  • Disease Reservoirs / virology*
  • HIV / immunology
  • HIV / isolation & purification
  • HIV / physiology
  • HIV Antibodies / immunology*
  • HIV Infections / immunology*
  • HIV Infections / virology*
  • Humans
  • Species Specificity
  • Viremia / immunology
  • Viremia / virology
  • Virion / metabolism
  • Virus Replication

Substances

  • Antibodies, Monoclonal
  • Antibodies, Neutralizing
  • HIV Antibodies