APOBEC3F determinants of HIV-1 Vif sensitivity

J Virol. 2014 Nov;88(21):12923-7. doi: 10.1128/JVI.02362-14. Epub 2014 Aug 20.

Abstract

HIV-1 Vif counteracts restrictive APOBEC3 proteins by targeting them for proteasomal degradation. To determine the regions mediating sensitivity to Vif, we compared human APOBEC3F, which is HIV-1 Vif sensitive, with rhesus APOBEC3F, which is HIV-1 Vif resistant. Rhesus-human APOBEC3F chimeras and amino acid substitution mutants were tested for sensitivity to HIV-1 Vif. This approach identified the α3 and α4 helices of human APOBEC3F as important determinants of the interaction with HIV-1 Vif.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Animals
  • Cytosine Deaminase / genetics
  • Cytosine Deaminase / metabolism*
  • DNA Mutational Analysis
  • HIV-1 / immunology*
  • Humans
  • Macaca mulatta
  • Mutant Proteins / genetics
  • Mutant Proteins / metabolism
  • Protein Interaction Mapping
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • vif Gene Products, Human Immunodeficiency Virus / metabolism*

Substances

  • Mutant Proteins
  • Recombinant Proteins
  • vif Gene Products, Human Immunodeficiency Virus
  • vif protein, Human immunodeficiency virus 1
  • APOBEC3F protein, human
  • Cytosine Deaminase