A Nile Red/BODIPY-based bimodal probe sensitive to changes in the micropolarity and microviscosity of the endoplasmic reticulum

Chem Commun (Camb). 2014 Oct 11;50(79):11672-5. doi: 10.1039/c4cc04915b.

Abstract

We herein report a fluorescent bimodal probe (1) capable of determining ER viscosity and polarity changes using FLIM and fluorescence ratiometry, respectively; during ER stress caused by tunicamycin, the viscosity was increased from ca. 129.5 to 182.0 cP and the polarity of the ER (dielectric constant, ε) enhanced from 18.5 to 21.1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Boron Compounds / chemistry
  • Boron Compounds / pharmacology*
  • Cell Polarity
  • Endoplasmic Reticulum / physiology*
  • Endoplasmic Reticulum Stress
  • Fluorescence
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / pharmacology*
  • HeLa Cells
  • Humans
  • Oxazines / chemistry
  • Oxazines / pharmacology*
  • Tunicamycin / pharmacology
  • Viscosity

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Boron Compounds
  • Fluorescent Dyes
  • Oxazines
  • Tunicamycin
  • nile red