Virus-host mucosal interactions during early SIV rectal transmission

Wuxun Lu; Fangrui Ma; Alexander Churbanov; Yanmin Wan; Yue Li; Guobin Kang; Zhe Yuan; Dong Wang; Chi Zhang; Jianqing Xu; Mark Lewis; Qingsheng Li

doi:10.1016/j.virol.2014.07.010

Virus-host mucosal interactions during early SIV rectal transmission

Virology. 2014 Sep:464-465:406-414. doi: 10.1016/j.virol.2014.07.010. Epub 2014 Aug 14.

Authors

Wuxun Lu¹, Fangrui Ma², Alexander Churbanov², Yanmin Wan³, Yue Li⁴, Guobin Kang¹, Zhe Yuan¹, Dong Wang⁵, Chi Zhang⁶, Jianqing Xu⁷, Mark Lewis⁸, Qingsheng Li⁹

Affiliations

¹ Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
² Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
³ Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology of MOE/MOH, Fudan University, Shanghai, China.
⁴ College of Life Sciences, Nankai University, Tianjin, China; Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
⁵ Department of Statistics, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
⁶ School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
⁷ Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology of MOE/MOH, Fudan University, Shanghai, China; State Key Laboratory for Infectious Disease Prevention and Control, China CDC, Beijing, China.
⁸ BIOQUAL, Inc., Rockville, MD 20850, USA.
⁹ Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA. Electronic address: qli@unl.edu.

Abstract

To deepen our understanding of early rectal transmission of HIV-1, we studied virus-host interactions in the rectal mucosa using simian immunodeficiency virus (SIV)-Indian rhesus macaque model and mRNA deep sequencing. We found that rectal mucosa actively responded to SIV as early as 3 days post-rectal inoculation (dpi) and mobilized more robust responses at 6 and 10 dpi. Our results suggest that the failure of the host to contain virus replication at the portal of entry is attributable to both a high-level expression of lymphocyte chemoattractant, proinflammatory and immune activation genes, which can recruit and activate viral susceptible target cells into mucosa; and a high-level expression of SIV accessory genes, which are known to be able to counter and evade host restriction factors and innate immune responses. This study provides new insights into the mechanism of rectal transmission.

Keywords: Mucosal response; Rectal transmission; Simian immunodeficiency virus (SIV); mRNA-seq.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Cytokines / genetics
Cytokines / immunology
Disease Models, Animal
HIV Infections / genetics
HIV Infections / immunology
HIV Infections / transmission*
HIV Infections / virology
HIV-1 / genetics
HIV-1 / physiology
Host-Pathogen Interactions
Humans
Intestinal Mucosa / virology*
Macaca mulatta
Male
Rectum / virology*
Simian Acquired Immunodeficiency Syndrome / genetics
Simian Acquired Immunodeficiency Syndrome / immunology
Simian Acquired Immunodeficiency Syndrome / transmission*
Simian Acquired Immunodeficiency Syndrome / virology
Simian Immunodeficiency Virus / genetics
Simian Immunodeficiency Virus / physiology*

Substances

Cytokines

Abstract

Publication types

MeSH terms

Substances

Grants and funding