Next generation sequencing technology: Advances and applications

H P J Buermans; J T den Dunnen

doi:10.1016/j.bbadis.2014.06.015

Next generation sequencing technology: Advances and applications

Biochim Biophys Acta. 2014 Oct;1842(10):1932-1941. doi: 10.1016/j.bbadis.2014.06.015. Epub 2014 Jul 1.

Authors

H P J Buermans¹, J T den Dunnen²

Affiliations

¹ Leiden Genome Technology Center, Leiden University Medical Center, Postbus 9600, 2300 RC Leiden, The Netherlands. Electronic address: h.buermans@lumc.nl.
² Leiden Genome Technology Center, Leiden University Medical Center, Postbus 9600, 2300 RC Leiden, The Netherlands. Electronic address: ddunnen@humgen.nl.

PMID: 24995601
DOI: 10.1016/j.bbadis.2014.06.015

Abstract

Impressive progress has been made in the field of Next Generation Sequencing (NGS). Through advancements in the fields of molecular biology and technical engineering, parallelization of the sequencing reaction has profoundly increased the total number of produced sequence reads per run. Current sequencing platforms allow for a previously unprecedented view into complex mixtures of RNA and DNA samples. NGS is currently evolving into a molecular microscope finding its way into virtually every fields of biomedical research. In this chapter we review the technical background of the different commercially available NGS platforms with respect to template generation and the sequencing reaction and take a small step towards what the upcoming NGS technologies will bring. We close with an overview of different implementations of NGS into biomedical research. This article is part of a Special Issue entitled: From Genome to Function.

Keywords: Applications; Basic technology; Nanopore; Next generation sequencing; Sequence by synthesis; Single molecule sequencing.

Publication types

Review