Development of a rapid diagnostic kit that uses an immunochromatographic device to detect antibodies in human sparganosis

Hiroshi Yamasaki; Takeshi Nakamura; Pewpan M Intapan; Wanchai Maleewong; Yasuyuki Morishima; Hiromu Sugiyama; Hiroyuki Matsuoka; Kaoru Kobayashi; Katsuyoshi Takayama; Yukuharu Kobayashi

doi:10.1128/CVI.00149-14

Development of a rapid diagnostic kit that uses an immunochromatographic device to detect antibodies in human sparganosis

Clin Vaccine Immunol. 2014 Sep;21(9):1360-3. doi: 10.1128/CVI.00149-14. Epub 2014 Jul 2.

Affiliations

¹ Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan hyamasak@niid.go.jp.
² Department of Parasitology, Kitasato University School of Medicine, Kanagawa, Japan.
³ Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
⁴ Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan.
⁵ Division of Medical Zoology, Jichi Medical University, Tochigi, Japan.
⁶ Division of Research and Development, Adtech Inc., Ltd., Oita, Japan.

Abstract

A diagnostic kit using an immunochromatographic device was developed to replace the time-consuming immunodiagnostic methods for human sparganosis. The kit was found to be faster and easier to use than an enzyme-linked immunosorbent assay (ELISA) and showed higher sensitivity and specificity. It will be useful for the laboratory diagnosis of hospitalized cases of sparganosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Helminth / blood*
Chromatography, Affinity / methods*
Diagnostic Tests, Routine / methods*
Humans
Sensitivity and Specificity
Sparganosis / diagnosis*
Sparganum / immunology*
Time Factors

Substances

Antibodies, Helminth