ImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas

PLoS One. 2014 Jun 20;9(6):e100342. doi: 10.1371/journal.pone.0100342. eCollection 2014.

Abstract

Objective: To develop a new ImmunoFISH technique for the study of oligodendrogliomas by combining a standard immunohistochemical stain using MIB-1 antibody with a standard FISH technique using commercial 1p36 and 19q13 chromosomal probes.

Methods: Validation was performed by two observers on a series of 36 pre-selected oligodendrogliomas and compared to the results previously determined by FISH alone.

Results: The ImFISH technique is easy to perform and to analyze and is no more time-consuming than the usual FISH technique. Our results show that the inter-observer reliability of ImFISH is high (κ = 0.86 and 0.95 respectively for 1p and 19q). Compared to FISH, the ImFISH exhibits a very high sensitivity (∼100%) and specificity (∼90%) for 1p and/or 19q deleted cases. The sensitivity is high for normal cases (∼85%) and imbalanced cases (∼90%) with a specificity ranging between 50 and 85%. Finally, there were no significant differences between FISH and ImFISH results calculated on 60, 40 or 20 cells.

Conclusion: Our study demonstrates the reliability of the ImFISH technique in oligodendrogliomas and emphasizes its advantage in poorly cellular tumoral specimen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Antinuclear / metabolism*
  • Antibodies, Monoclonal / metabolism*
  • Brain Neoplasms / genetics*
  • Brain Neoplasms / metabolism
  • Chromosomes, Human, Pair 1 / genetics*
  • Chromosomes, Human, Pair 1 / metabolism
  • Chromosomes, Human, Pair 19 / genetics*
  • Chromosomes, Human, Pair 19 / metabolism
  • Female
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence / methods*
  • Male
  • Middle Aged
  • Observer Variation
  • Oligodendroglioma / genetics*
  • Oligodendroglioma / metabolism
  • Reproducibility of Results

Substances

  • Antibodies, Antinuclear
  • Antibodies, Monoclonal
  • MIB-1 antibody

Grants and funding

This study was supported by the Fondation des hôpitaux Enfant-Jésus – Saint Sacrement (Quebec city - Quebec): grant number 682/8317. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.