Minimally invasive approach to the repair of injured skeletal muscle with a shape-memory scaffold

Mol Ther. 2014 Aug;22(8):1441-1449. doi: 10.1038/mt.2014.78. Epub 2014 Apr 28.

Abstract

Repair of injured skeletal muscle by cell therapies has been limited by poor survival of injected cells. Use of a carrier scaffold delivering cells locally, may enhance in vivo cell survival, and promote skeletal muscle regeneration. Biomaterial scaffolds are often implanted into muscle tissue through invasive surgeries, which can result in trauma that delays healing. Minimally invasive approaches to scaffold implantation are thought to minimize these adverse effects. This hypothesis was addressed in the context of a severe mouse skeletal muscle injury model. A degradable, shape-memory alginate scaffold that was highly porous and compressible was delivered by minimally invasive surgical techniques to injured tibialis anterior muscle. The scaffold controlled was quickly rehydrated in situ with autologous myoblasts and growth factors (either insulin-like growth factor-1 (IGF-1) alone or IGF-1 with vascular endothelial growth factor (VEGF)). The implanted scaffolds delivering myoblasts and IGF-1 significantly reduced scar formation, enhanced cell engraftment, and improved muscle contractile function. The addition of VEGF to the scaffold further improved functional recovery likely through increased angiogenesis. Thus, the delivery of myoblasts and dual local release of VEGF and IGF-1 from degradable scaffolds implanted through a minimally invasive procedure effectively promoted the functional regeneration of injured skeletal muscle.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alginates / chemistry
  • Animals
  • Biocompatible Materials
  • Cell Proliferation
  • Cell Survival
  • Disease Models, Animal
  • Glucuronic Acid / chemistry
  • Hexuronic Acids / chemistry
  • Insulin-Like Growth Factor I / metabolism
  • Male
  • Mice
  • Mice, Inbred C3H
  • Muscle, Skeletal / injuries*
  • Muscle, Skeletal / physiopathology
  • Muscle, Skeletal / surgery*
  • Myoblasts, Skeletal / metabolism
  • Myoblasts, Skeletal / transplantation*
  • Soft Tissue Injuries / therapy
  • Tissue Scaffolds / chemistry
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • Alginates
  • Biocompatible Materials
  • Hexuronic Acids
  • Vascular Endothelial Growth Factor A
  • insulin-like growth factor-1, mouse
  • vascular endothelial growth factor A, mouse
  • Insulin-Like Growth Factor I
  • Glucuronic Acid