Analysis of structural heterogeneity of fucoidan from Hizikia fusiforme by ES-CID-MS/MS

Peipei Wang; Xiaoliang Zhao; Youjing Lv; Yanan Liu; Yinzhi Lang; Jiandong Wu; Xin Liu; Miaomiao Li; Guangli Yu

doi:10.1016/j.carbpol.2012.05.084

Analysis of structural heterogeneity of fucoidan from Hizikia fusiforme by ES-CID-MS/MS

Carbohydr Polym. 2012 Sep 1;90(1):602-7. doi: 10.1016/j.carbpol.2012.05.084. Epub 2012 May 31.

Authors

Peipei Wang¹, Xiaoliang Zhao¹, Youjing Lv¹, Yanan Liu¹, Yinzhi Lang¹, Jiandong Wu¹, Xin Liu², Miaomiao Li², Guangli Yu¹

Affiliations

¹ Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
² Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.

PMID: 24751082
DOI: 10.1016/j.carbpol.2012.05.084

Abstract

A fucoidan from the brown alga Hizikia fusiforme was extracted with hot water and fractionated by anion-exchange chromatography. The main fraction YF5 was depolymerized by partial acid hydrolysis and further purified by gel filtration chromatograph. The sequences of oligosaccharides generated were then determined by ES-CID-MS/MS, which gave directly information on the structural heterogeneity of fucoidan from H. fusiforme. It was shown that YF5 had a backbone of a repeating disaccharide unit of [→4GlcAβ1,2Manα1→], in good agreement with previous report by NMR and methylation analysis. In the product-ion spectra, the unique (0.2)A type fragmentation was important to establish the presence of a 2-linked Man in the backbone structure. In addition, abundant novel heterogeneous branched structural fragments were also detected and characterized: Fuc1,3Fuc, Fuc1,3Gal, Fuc1,4GlcA, Gal1,4Gal, Gal1,4GlcA and GlcA1,4GlcA. The sulfation mainly occurred at C2 or C4 of the fucose residue and C2, C4 or C6 of the galactose residue.

Publication types

Research Support, Non-U.S. Gov't