Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains

Liang Chen; Kalyan D Chavda; Jacqueline Findlay; Gisele Peirano; Katie Hopkins; Johann D D Pitout; Robert A Bonomo; Neil Woodford; Frank R DeLeo; Barry N Kreiswirth

doi:10.1128/AAC.02673-14

Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains

Antimicrob Agents Chemother. 2014 Jul;58(7):4196-9. doi: 10.1128/AAC.02673-14. Epub 2014 Apr 14.

Authors

Affiliations

¹ Public Health Research Institute Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
² Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, Public Health England, London, United Kingdom.
³ Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada Departments of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada.
⁴ Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada Departments of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada Departments of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada.
⁵ Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA Departments of Medicine, Pharmacology, Molecular Biology, and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA.
⁶ Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.
⁷ Public Health Research Institute Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA kreiswba@njms.rutgers.edu.

Abstract

We developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258] cps-1 and cps-2) in epidemic Klebsiella pneumoniae ST258 strains. The assay performed with excellent sensitivity (100%) and specificity (100%) for identifying cps types in 60 ST258 K. pneumoniae sequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association between cps type and K. pneumoniae carbapenemase (KPC) variant: cps-1 is largely associated with KPC-2, while cps-2 is primarily associated with KPC-3.

Publication types

Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Capsules / classification
Bacterial Capsules / genetics*
Bacterial Proteins / biosynthesis*
Bacterial Proteins / genetics
Carbapenems / pharmacology
DNA, Bacterial / genetics
Drug Resistance, Bacterial / genetics*
Humans
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / isolation & purification
Klebsiella pneumoniae / pathogenicity
Microbial Sensitivity Tests
Molecular Sequence Data
Molecular Typing
Multiplex Polymerase Chain Reaction
beta-Lactamases / biosynthesis*
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
Bacterial Proteins
Carbapenems
DNA, Bacterial
beta-Lactamases
beta-lactamase KPC-3, Klebsiella pneumoniae
carbapenemase-2, Klebsiella pneumoniae

Associated data

GENBANK/KF793262
GENBANK/KF793263

Abstract

Publication types

MeSH terms

Substances

Associated data

Grants and funding