Sequence-level and dual-phase identification of Salmonella flagellum antigens by liquid chromatography-tandem mass spectrometry (LC-MS/MS)

J Clin Microbiol. 2014 Jun;52(6):2189-92. doi: 10.1128/JCM.00242-14. Epub 2014 Apr 2.

Abstract

Forty-three reference strains involving the 24 most common serovars of Salmonella enterica were examined by using a mass spectrometry-based H antigen typing platform (MS-H). The results indicate that MS-H can be used as a sensitive, rapid, and straightforward approach for the typing of Salmonella flagella at the molecular level without antiserum and phase inversion.

MeSH terms

  • Antigens, Bacterial / chemistry*
  • Bacteriological Techniques / methods
  • Chromatography, Liquid / methods*
  • Flagella / chemistry*
  • Humans
  • Salmonella enterica / chemistry*
  • Salmonella enterica / classification*
  • Tandem Mass Spectrometry / methods*

Substances

  • Antigens, Bacterial
  • H antigen