Nectar secretion requires sucrose phosphate synthases and the sugar transporter SWEET9

Nature. 2014 Apr 24;508(7497):546-9. doi: 10.1038/nature13082. Epub 2014 Mar 16.

Abstract

Angiosperms developed floral nectaries that reward pollinating insects. Although nectar function and composition have been characterized, the mechanism of nectar secretion has remained unclear. Here we identify SWEET9 as a nectary-specific sugar transporter in three eudicot species: Arabidopsis thaliana, Brassica rapa (extrastaminal nectaries) and Nicotiana attenuata (gynoecial nectaries). We show that SWEET9 is essential for nectar production and can function as an efflux transporter. We also show that sucrose phosphate synthase genes, encoding key enzymes for sucrose biosynthesis, are highly expressed in nectaries and that their expression is also essential for nectar secretion. Together these data are consistent with a model in which sucrose is synthesized in the nectary parenchyma and subsequently secreted into the extracellular space via SWEET9, where sucrose is hydrolysed by an apoplasmic invertase to produce a mixture of sucrose, glucose and fructose. The recruitment of SWEET9 for sucrose export may have been a key innovation, and could have coincided with the evolution of core eudicots and contributed to the evolution of nectar secretion to reward pollinators.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alkyl and Aryl Transferases / metabolism
  • Animals
  • Arabidopsis / cytology
  • Arabidopsis / enzymology
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / metabolism
  • Brassica rapa / anatomy & histology
  • Brassica rapa / enzymology
  • Brassica rapa / metabolism
  • Carbohydrate Metabolism
  • Extracellular Space / metabolism
  • Flowers / physiology
  • Glucosyltransferases / genetics
  • Glucosyltransferases / metabolism*
  • HEK293 Cells
  • Humans
  • Membrane Transport Proteins / metabolism
  • Nicotiana / anatomy & histology
  • Nicotiana / enzymology
  • Nicotiana / metabolism
  • Oocytes
  • Plant Nectar / biosynthesis
  • Plant Nectar / metabolism*
  • Plant Proteins / metabolism*
  • Pollination
  • Protein Transport
  • Sequence Homology
  • Starch / metabolism
  • Sucrose / metabolism*
  • Xenopus
  • beta-Fructofuranosidase / metabolism

Substances

  • Arabidopsis Proteins
  • Membrane Transport Proteins
  • Plant Nectar
  • Plant Proteins
  • SWEET12 protein, Arabidopsis
  • Sucrose
  • Starch
  • Glucosyltransferases
  • sucrose-phosphate synthase
  • Alkyl and Aryl Transferases
  • SPS1 protein, Arabidopsis
  • SPS2 protein, Arabidopsis
  • beta-Fructofuranosidase