Detection of anti-cyclic citrullinated peptide using a time-resolved fluoroimmunoassay

J Immunoassay Immunochem. 2014;35(3):221-32. doi: 10.1080/15321819.2013.841193.

Abstract

In an effort to enhance the linear range of anti-CCP we developed a new immunoassay based on time-resolved fluoroimmunoassay. The precision, sensitivity, specificity, and stability of the assay were evaluated ELISA set as control. The anti-CCP IgG TRFIA kit we established had a wider detectable range than commercial ELISA ones. With regard to intra- and inter-assay precision, the TRFIA kit was better than threee commercial ELISA ones. The mean recovery rate was 101.0%. The TRFIA we developed for anti-CCP IgG detection yielded a more sensitive and reliable method for RA diagnosis and large-scale screening programs as well.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arthritis, Rheumatoid / blood
  • Arthritis, Rheumatoid / diagnosis*
  • Arthritis, Rheumatoid / immunology
  • Autoantibodies / blood*
  • Calibration
  • Enzyme-Linked Immunosorbent Assay
  • Europium / chemistry
  • Fluoroimmunoassay / methods*
  • Fluoroimmunoassay / standards
  • Humans
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / chemistry
  • Immunoglobulin G / isolation & purification
  • Mice
  • Peptides, Cyclic / blood
  • Peptides, Cyclic / immunology*
  • Reagent Kits, Diagnostic
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Staining and Labeling / methods

Substances

  • Autoantibodies
  • Immunoglobulin G
  • Peptides, Cyclic
  • Reagent Kits, Diagnostic
  • cyclic citrullinated peptide
  • Europium