Get your high-quality low-cost genome sequence

Luigi Faino; Bart P H J Thomma

doi:10.1016/j.tplants.2014.02.003

Get your high-quality low-cost genome sequence

Trends Plant Sci. 2014 May;19(5):288-91. doi: 10.1016/j.tplants.2014.02.003. Epub 2014 Mar 11.

Authors

Luigi Faino¹, Bart P H J Thomma²

Affiliations

¹ Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
² Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands. Electronic address: bart.thomma@wur.nl.

PMID: 24636622
DOI: 10.1016/j.tplants.2014.02.003

Abstract

The study of whole-genome sequences has become essential for almost all branches of biological research. Next-generation sequencing (NGS) has revolutionized the scalability, speed, and resolution of sequencing and brought genomic science within reach of academic laboratories that study non-model organisms. Here, we show that a high-quality draft genome of a eukaryote can be obtained at relatively low cost by exploiting a hybrid combination of sequencing strategies.

Keywords: eukaryote; fungal genome; genome structure; hybrid assembly; next-generation sequencing (NGS); repetitive element.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Library
Genome, Plant / genetics*
High-Throughput Nucleotide Sequencing / economics
High-Throughput Nucleotide Sequencing / methods*
Plants / genetics*
Sequence Analysis, DNA / economics
Sequence Analysis, DNA / methods
Sequence Analysis, DNA / standards
Systems Biology*