Biological imaging with coherent Raman scattering microscopy: a tutorial

J Biomed Opt. 2014 Jul;19(7):71407. doi: 10.1117/1.JBO.19.7.071407.

Abstract

Coherent Raman scattering (CRS) microscopy is gaining acceptance as a valuable addition to the imaging toolset of biological researchers. Optimal use of this label-free imaging technique benefits from a basic understanding of the physical principles and technical merits of the CRS microscope. This tutorial offers qualitative explanations of the principles behind CRS microscopy and provides information about the applicability of this nonlinear optical imaging approach for biological research.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Equipment Design
  • Humans
  • Image Processing, Computer-Assisted / methods*
  • Lasers
  • Light
  • Meibomian Glands / pathology
  • Mice
  • Microscopy / methods*
  • Microscopy, Fluorescence / methods
  • Optics and Photonics
  • Photons
  • Scattering, Radiation
  • Spectrum Analysis, Raman / methods*
  • Vibration