When working with hyperpolarized species, it is often difficult to maintain a stable level of magnetization over consecutive experiments, which renders their detection at the trace level cumbersome, even when combined with chemical exchange saturation transfer (CEST). We report herein the use of ultra-fast Z-spectroscopy as a powerful means to detect low concentrations of 129Xe NMR-based sensors and to measure the in-out xenon exchange. Modifications of the original sequence enable a multiplexed detection of several sensors, as well as the extraction of the exchange buildup rate constant in a single-shot fashion.
Keywords: Biosensors; Exchange; Hyperpolarization; Magnetic Resonance; Sensitivity.