[Non-invasive fetal RHD genotyping: Validation of the method with 200 patients]

Abstract

Background: Non-invasive fetal RHD genotyping is an important tool to assess the risk of fetuse's hemolytic disease of anti-D allo-immunized pregnant woman by non-invasive method. A method of genotyping has been developed in the laboratory of Lyon-GHE according to Minon's team (J Gynecol Obstet Biol Reprod 2005): exon 4, 5, and 10 are amplified by real time PCR. At first, genotyping results of 200 pregnant women have been compared with RH1 phenotype at birth. The most important parameters of validation have been tested: the sensibility and the specificity; the negative predictive value; the correlation study permitted to define criteria of biological interpretation. The validation of this method permitted to determine critical points and the limits of the method due to the minor amount of fetal DNA in the maternal plasma and existence of many variant forms of the RHD gene.

Conclusion: We worked too in the perspective to the accreditation for our genetic laboratory.

Keywords: Accreditation; Accréditation; Allo-immunisation; Allo-immunization; Diagnostic prénatal; Génotypage par méthode non invasive; Non-invasive genotyping; Phénotype RH1; Prenatal diagnosis; RH1 phenotype; Validation de méthode; Validation of the method.