Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP

Nucleic Acids Res. 2014 Apr;42(8):5059-71. doi: 10.1093/nar/gku095. Epub 2014 Feb 7.

Abstract

The nuclear phase of the gene expression pathway culminates in the export of mature messenger RNAs (mRNAs) to the cytoplasm through nuclear pore complexes. GANP (germinal- centre associated nuclear protein) promotes the transfer of mRNAs bound to the transport factor NXF1 to nuclear pore complexes. Here, we demonstrate that GANP, subunit of the TRanscription-EXport-2 (TREX-2) mRNA export complex, promotes selective nuclear export of a specific subset of mRNAs whose transport depends on NXF1. Genome-wide gene expression profiling showed that half of the transcripts whose nuclear export was impaired following NXF1 depletion also showed reduced export when GANP was depleted. GANP-dependent transcripts were highly expressed, yet short-lived, and were highly enriched in those encoding central components of the gene expression machinery such as RNA synthesis and processing factors. After injection into Xenopus oocyte nuclei, representative GANP-dependent transcripts showed faster nuclear export kinetics than representative transcripts that were not influenced by GANP depletion. We propose that GANP promotes the nuclear export of specific classes of mRNAs that may facilitate rapid changes in gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / physiology*
  • Active Transport, Cell Nucleus
  • Animals
  • Cell Line, Tumor
  • Cell Nucleus / metabolism*
  • Humans
  • Intracellular Signaling Peptides and Proteins / physiology*
  • Nucleocytoplasmic Transport Proteins / physiology
  • RNA, Messenger / classification
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / physiology
  • Xenopus

Substances

  • Intracellular Signaling Peptides and Proteins
  • NXF1 protein, human
  • Nucleocytoplasmic Transport Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • Acetyltransferases
  • MCM3AP protein, human

Associated data

  • GEO/GSE54481