Synthesis, characterization, and in vitro studies of graphene oxide/chitosan-polyvinyl alcohol films

Andreea Madalina Pandele; Mariana Ionita; Livia Crica; Sorina Dinescu; Marieta Costache; Horia Iovu

doi:10.1016/j.carbpol.2013.10.085

Synthesis, characterization, and in vitro studies of graphene oxide/chitosan-polyvinyl alcohol films

Carbohydr Polym. 2014 Feb 15:102:813-20. doi: 10.1016/j.carbpol.2013.10.085. Epub 2013 Nov 5.

Authors

Andreea Madalina Pandele¹, Mariana Ionita², Livia Crica¹, Sorina Dinescu³, Marieta Costache³, Horia Iovu¹

Affiliations

¹ University Politehnica of Bucharest, 132 Calea Grivitei, 010737 Bucharest, Romania.
² University Politehnica of Bucharest, 132 Calea Grivitei, 010737 Bucharest, Romania. Electronic address: mariana.ionita@polimi.it.
³ University of Bucharest, Splaiul Independentei 91-95, 050095 Bucharest, Romania.

PMID: 24507351
DOI: 10.1016/j.carbpol.2013.10.085

Abstract

Nanocomposites based on chitosan-polyvinyl alcohol (CS-PVA) and graphene oxide (GO) were prepared by casting the stable aqueous mixture of the components. SEM, TEM and X-ray diffraction showed that graphene oxide is largely dispersed on molecular scale within CS-PVA matrix. FTIR investigation indicated the occurrence of some interaction between graphene oxide nanosheets and CS-PVA. The obtained composites are mechanically strong and exhibit improved thermal stability. By addition of 6 wt.% GO within CS-PVA blend, the elastic modulus increased over 200%. The cell viability and proliferation results showed that MC3T3-E1 mouse osteoblastic cells can adhere and developed on the CS-PVA/GO composite films. A significant proliferation potential was displayed by the cells in contact with CS-PVA/GO 6 wt.%. Graphene oxide reinforced CS-PVA with high mechanical and bioactive properties are potential candidates for tissue engineering.

Keywords: Cells culture; Chitosan–polyvinyl alcohol; Graphene oxide; Mechanical strength; Nanocomposite.