Macromolecular crowding meets tissue engineering by self-assembly: a paradigm shift in regenerative medicine

Adv Mater. 2014 May 21;26(19):3024-34. doi: 10.1002/adma.201304428. Epub 2014 Feb 6.

Abstract

MMC, the addition of inert polydispersed macromolecules in the culture media, effectively emulates the dense in vivo extracellular space, resulting in amplified deposition of ECM in vitro and subsequent production of cohesive, ECM-rich living substitutes.

Keywords: cell-sheet tissue engineering; excluding volume effect; extracellular matrix deposition; macromolecular crowding; macromolecular polydispersity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylic Resins / chemistry
  • Cell Line
  • Collagen / metabolism
  • Dextran Sulfate / chemistry
  • Extracellular Matrix / chemistry
  • Extracellular Matrix / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Fibroblasts / pathology
  • Humans
  • Macromolecular Substances / chemistry*
  • Matrix Metalloproteinase 2 / metabolism
  • Regenerative Medicine*
  • Tissue Engineering*

Substances

  • Acrylic Resins
  • Macromolecular Substances
  • poly-N-isopropylacrylamide
  • Collagen
  • Dextran Sulfate
  • Matrix Metalloproteinase 2