Xpert GBS assay for rapid detection of group B streptococcus in gastric fluid samples from newborns

J Clin Microbiol. 2014 Feb;52(2):657-9. doi: 10.1128/JCM.02532-13. Epub 2013 Nov 27.

Abstract

The Xpert GBS real-time PCR assay was applied to gastric fluid samples from 143 newborns, and it detected group B streptococcus (GBS) within 1 h for 16 (11.2%) cases, while microscopic examination detected only 2 cases. The sensitivity and specificity of the Xpert GBS were 80% and 100%, respectively, with regard to 20 cases of GBS colonization or infection. Concordance of Xpert GBS results versus culture was 92.3%. This test detects in a timely manner newborns at risk for invasive GBS disease.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Bacteriological Techniques / methods*
  • Female
  • Gastric Juice / microbiology*
  • Humans
  • Infant, Newborn
  • Microscopy / methods
  • Molecular Diagnostic Techniques / methods*
  • Pregnancy
  • Prospective Studies
  • Real-Time Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Streptococcal Infections / diagnosis*
  • Streptococcus agalactiae / isolation & purification*
  • Time