Inhibition of SATB1 by shRNA suppresses the proliferation of cutaneous malignant melanoma

Cancer Biother Radiopharm. 2014 Mar;29(2):77-82. doi: 10.1089/cbr.2013.1502. Epub 2014 Jan 7.

Abstract

Overexpression of special AT-rich sequence binding protein 1 (SATB1), a global genome organizer, as a predictor of poor prognosis in cutaneous malignant melanoma (CMM) attracted great interest in previous research. In this article, upregulated SATB1 was observed in three melanoma cell lines and in eight CMM tissues. After downregulating SATB1 by RNAi, proliferation of A375 was suppressed in vitro and in vivo. In summary, the proliferation of CMM could be related closely to the SATB1 gene. Interference of this gene may be a promising method for CMM therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Growth Processes / genetics
  • Cell Line, Tumor
  • Down-Regulation
  • Female
  • Gene Silencing
  • Humans
  • Matrix Attachment Region Binding Proteins / antagonists & inhibitors*
  • Matrix Attachment Region Binding Proteins / genetics
  • Melanoma / genetics
  • Melanoma / pathology
  • Melanoma / therapy*
  • Melanoma, Cutaneous Malignant
  • Mice, Nude
  • RNA, Small Interfering / administration & dosage*
  • RNA, Small Interfering / genetics
  • Random Allocation
  • Skin Neoplasms
  • Transfection
  • Up-Regulation
  • Xenograft Model Antitumor Assays

Substances

  • Matrix Attachment Region Binding Proteins
  • RNA, Small Interfering
  • SATB1 protein, human