Bidirectional homeostatic plasticity induced by interneuron cell death and transplantation in vivo

Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):492-7. doi: 10.1073/pnas.1307784111. Epub 2013 Dec 16.

Abstract

Chronic changes in excitability and activity can induce homeostatic plasticity. These perturbations may be associated with neurological disorders, particularly those involving loss or dysfunction of GABA interneurons. In distal-less homeobox 1 (Dlx1(-/-)) mice with late-onset interneuron loss and reduced inhibition, we observed both excitatory synaptic silencing and decreased intrinsic neuronal excitability. These homeostatic changes do not fully restore normal circuit function, because synaptic silencing results in enhanced potential for long-term potentiation and abnormal gamma oscillations. Transplanting medial ganglionic eminence interneuron progenitors to introduce new GABAergic interneurons, we demonstrate restoration of hippocampal function. Specifically, miniature excitatory postsynaptic currents, input resistance, hippocampal long-term potentiation, and gamma oscillations are all normalized. Thus, in vivo homeostatic plasticity is a highly dynamic and bidirectional process that responds to changes in inhibition.

Keywords: LTP; excitatory/inhibitory balance; gamma frequency oscillations; neural transplantation.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Death
  • Cell Transplantation
  • Electrophysiology
  • Excitatory Postsynaptic Potentials / physiology
  • GABAergic Neurons / metabolism
  • Gene Silencing
  • Green Fluorescent Proteins / metabolism
  • Hippocampus / metabolism
  • Homeodomain Proteins / genetics*
  • Homeostasis
  • Immunohistochemistry
  • Interneurons / metabolism
  • Interneurons / pathology*
  • Long-Term Potentiation
  • Male
  • Mice
  • Neural Stem Cells / transplantation*
  • Neuronal Plasticity*
  • Neurons / metabolism
  • Oscillometry
  • Synapses / physiology
  • Synaptic Transmission / physiology
  • Transcription Factors / genetics*
  • gamma-Aminobutyric Acid / metabolism

Substances

  • Distal-less homeobox proteins
  • Homeodomain Proteins
  • Transcription Factors
  • Green Fluorescent Proteins
  • gamma-Aminobutyric Acid