Objective: To investigate the effect of salt stress on DNA methylation in Isatis indigotica.
Methods: Methylation sensitive amplification polymorphism (MSAP) approach was used in this study.
Results: By using 15 pairs of selective primers combinations, a total of 632 MSAP bands were obtained in Isatis indigotica genome under salt stress. In all, 44 (6.9%) CCGG sites displayed cytosine methylation changes under salt stress. Among these sites, 31(4.9%) CCGG sites underwent hypermethylation changes and 13 (2.0%) CCGG sites underwent demethylation changes.
Conclusion: This result provides evidence for DNA methylation remodeling occurring immediately after salt stress affects Isatis indigotica.