Metallic tip enhanced fluorescence for DNA replication monitoring

Annu Int Conf IEEE Eng Med Biol Soc. 2013:2013:488-91. doi: 10.1109/EMBC.2013.6609543.

Abstract

We have successfully performed localized loop-mediated isothermal reactions of hepatitis B virus (HBV) and hepatitis C virus (HCV) on the apex (50~100 nm) of metallic tips coated with Bst polymerases. The SYBR green molecules binding to the new formed HBV DNA inside the optical near fields were excited by two-photon fluorescence microscopy, and directly imaged in far field. Another reporter primer is used for HCV replication detection. Preliminary results are presented in this manuscript.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers / genetics
  • DNA Replication
  • DNA, Viral / genetics*
  • DNA-Directed DNA Polymerase / chemistry
  • Enzymes, Immobilized / chemistry
  • Fluorescence
  • Hepacivirus / genetics
  • Hepatitis B virus / genetics
  • Humans
  • Kinetics
  • Microscopy, Fluorescence, Multiphoton
  • Molecular Diagnostic Techniques / instrumentation*
  • Nucleic Acid Amplification Techniques / instrumentation*

Substances

  • DNA Primers
  • DNA, Viral
  • Enzymes, Immobilized
  • DNA-Directed DNA Polymerase