Epstein-Barr virus and human herpesvirus 6 detection in a non-Hodgkin's diffuse large B-cell lymphoma cohort by using RNA sequencing

J Virol. 2013 Dec;87(23):13059-62. doi: 10.1128/JVI.02380-13. Epub 2013 Sep 18.

Abstract

Comprehensive virome analysis of RNA sequence (RNA-seq) data sets from 118 non-Hodgkin's B-cell lymphomas revealed a small subset that is positive for Epstein-Barr virus (EBV) or human herpesvirus 6B (HHV-6B), with one coinfection. EBV transcriptome analysis revealed expression of the latency genes RPMS1, LMP1, and LMP2, with one sample additionally showing a high level of early lytic expression and another sample showing a high level of EBNA2 expression. HHV-6B transcriptome analysis revealed that the majority of genes were transcribed.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cohort Studies
  • Epstein-Barr Virus Infections / diagnosis
  • Epstein-Barr Virus Infections / virology*
  • Gene Expression Regulation, Viral
  • Herpesvirus 4, Human / genetics
  • Herpesvirus 4, Human / isolation & purification*
  • Herpesvirus 6, Human / genetics
  • Herpesvirus 6, Human / isolation & purification*
  • Humans
  • Lymphoma, Large B-Cell, Diffuse / diagnosis
  • Lymphoma, Large B-Cell, Diffuse / virology*
  • Roseolovirus Infections / diagnosis
  • Roseolovirus Infections / virology*
  • Sequence Analysis, RNA
  • Viral Proteins / genetics*

Substances

  • Viral Proteins