Objective: To establish the HPLC fingerprint of Gymnadenia conopsea from different regions of Qinghai-Tibet Plateau.
Methods: 12 batches of Gymnadenia conopsea were measured by the RP-HPLC, and their fingerprints were obtained. Chromatographic condition: The sample was separated on column of Kromasil C18 (4.6 mm x 250 mm,5 microm) and gradiently eluted with a mixture of methanol and water (containing 0.04% phosphoric acid). The flow rate was 0.7 mL/min. The detection wavelength was set at 222 nm and the column temperature was 30 degrees C.
Results: The HPLC fingerprint of Gymnadenia conopsea was set up and 13 common peaks were selected,the results of method validation met technical requirement of fingerprint,the similarity of 12 batches Gymnadenia conopsea was 0.904 - 0.989. Principal components analysis and clustering analysis were used in the identification of the samples.
Conclusion: The method is simple, practicable and reliable, which can be used for the quality control of Gymnadenia conopsea.