The role of NEFL in cell growth and invasion in head and neck squamous cell carcinoma cell lines

J Oral Pathol Med. 2014 Mar;43(3):191-8. doi: 10.1111/jop.12109. Epub 2013 Aug 31.

Abstract

The neurofilament light polypeptide (NEFL) gene located on chromosome 8q21 is associated with the cancer of several organs and is regarded as a potential tumor suppressor gene. However, the role of the NEFL protein has not yet been studied in cancer cells. Although evidence suggests that there is a correlation between NEFL expression and cancer, studies regarding the role of the NEFL protein have been mostly limited to neurological diseases, such as Charcot-Marie-Tooth's disease (CMT). Most of these studies have not explored the role of NEFL in cancer cell apoptosis and/or invasion. In this study, NEFL expression was manipulated, and apoptosis and invasion were compared in head and neck squamous cell carcinoma cell lines. The results show that the expression of NEFL induces cancer cell apoptosis and inhibits invasion in these cell lines, suggesting that NEFL may play a role in cancer cell apoptosis and invasion.

Keywords: NEFL; growth; head and neck squamous cell carcinoma; invasion.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / physiology
  • Carcinogenesis / pathology
  • Carcinoma, Squamous Cell / pathology*
  • Caspase 3 / analysis
  • Cell Line, Tumor
  • Cell Proliferation
  • Gene Silencing
  • Genetic Vectors / genetics
  • Head and Neck Neoplasms / pathology*
  • Humans
  • Neoplasm Invasiveness
  • Neurofilament Proteins / genetics
  • Neurofilament Proteins / physiology*
  • RNA, Small Interfering
  • Transfection

Substances

  • Neurofilament Proteins
  • RNA, Small Interfering
  • neurofilament protein L
  • CASP3 protein, human
  • Caspase 3