Autonomous molecular cascades for evaluation of cell surfaces

Nat Nanotechnol. 2013 Aug;8(8):580-6. doi: 10.1038/nnano.2013.142. Epub 2013 Jul 28.

Abstract

Molecular automata are mixtures of molecules that undergo precisely defined structural changes in response to sequential interactions with inputs. Previously studied nucleic acid-based automata include game-playing molecular devices (MAYA automata) and finite-state automata for the analysis of nucleic acids, with the latter inspiring circuits for the analysis of RNA species inside cells. Here, we describe automata based on strand-displacement cascades directed by antibodies that can analyse cells by using their surface markers as inputs. The final output of a molecular automaton that successfully completes its analysis is the presence of a unique molecular tag on the cell surface of a specific subpopulation of lymphocytes within human blood cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies / metabolism
  • Blood Cells / chemistry*
  • Cell Differentiation / physiology
  • Cell Lineage / physiology
  • Cell Surface Display Techniques*
  • Computers, Molecular
  • Humans
  • Streptavidin / metabolism
  • T-Lymphocytes / metabolism

Substances

  • Antibodies
  • Streptavidin