Effect of chronic kidney disease on the healing of titanium implants

Bone. 2013 Oct;56(2):410-5. doi: 10.1016/j.bone.2013.07.014. Epub 2013 Jul 19.

Abstract

Chronic kidney disease (CKD) has become a worldwide public health problem. However, its effect on osseointegration of dental implants is largely unknown. The aim of this study is to investigate whether CKD impairs the quality of the osseointegration of titanium implants. Uremia was induced by 5/6 nephrectomy in mice, and serum levels of BUN, FGF23, PTH and ALP were significantly increased. For in vitro tests, bone marrow mesenchymal stem cells (BMMSCs) were obtained and cultured on titanium discs. There was no significant difference in term of expression of osteogenic marker genes including Osx, Col-1, Ocn, and Opn, as quantified by qPCR. Moreover, Alizarin Red S staining showed comparable mineralized nodules formation. Histomorphometrical analysis of experimental implants inserted in the femurs of CKD mice revealed a trend of decreased BIC ratio at 2-week healing. The strength of bone-implant integration, as measured by a push-in method, was significantly lower for the CKD group at 2 weeks, although a comparable level was reached at 4 weeks. These results demonstrated that CKD only negatively affects the osseointegration of titanium implants at the early stage.

Keywords: Bone mineralization; Chronic kidney diseases; Dental implants; Fibroblast growth factor 23 (FGF23); Osseointegration; Parathyroid hormone (PTH).

MeSH terms

  • Animals
  • Dental Implants*
  • Female
  • Fibroblast Growth Factor-23
  • Fibroblast Growth Factors / blood
  • Mice
  • Mice, Inbred C57BL
  • Osseointegration / physiology*
  • Parathyroid Hormone / blood
  • Renal Insufficiency, Chronic / blood
  • Renal Insufficiency, Chronic / physiopathology*
  • Titanium*
  • Uremia / physiopathology

Substances

  • Dental Implants
  • Fgf23 protein, mouse
  • Parathyroid Hormone
  • Fibroblast Growth Factors
  • Fibroblast Growth Factor-23
  • Titanium