SIRT3 expression in hepatocellular carcinoma and its impact on proliferation and invasion of hepatoma cells

Asian Pac J Trop Med. 2013 Aug;6(8):649-52. doi: 10.1016/S1995-7645(13)60112-1.

Abstract

Objective: To observe expression of SIRT3 in normal liver tissue, cirrhotic tissue and hepatocellular carcinoma (HCC) tissues, and to explore the significance of SIRT3 in primary HCC.

Methods: SIRT3 expression was detected in 10 normal cases, 30 cases with, 30 HCC cases by immunohistochemical and Western-blotting method.

Results: Immunohistochemical assay showed that the SIRT3 positive expression rates were 100.0% (10/10), 96.7% (29/30) and 60.0% (18/30), respectively in normal group, paracancer group and HCC group. And the SIRT3 expression in HCC group was significantly lower than in normal group and paracancer group (P<0.05). Western-blotting showed the SIRT3 expression in cancer tissue was 0.29±0.07, significantly lower than that in paracancer group and normal group (P<0.05). SIRT3 expression was related to the differentiation degree and portal vein tumor thrombus (P<0.05).

Conclusions: Abnormal expression of SIRT3 is closely related to the biological behavior of primary HCC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Carcinoma, Hepatocellular / pathology*
  • Cell Proliferation*
  • Female
  • Hepatocytes / physiology*
  • Humans
  • Immunohistochemistry
  • Liver / pathology
  • Liver Cirrhosis / pathology
  • Liver Neoplasms / pathology*
  • Male
  • Middle Aged
  • Neoplasm Invasiveness / pathology*
  • Sirtuin 3 / biosynthesis*

Substances

  • SIRT3 protein, human
  • Sirtuin 3