3-D stimulated emission depletion microscopy with programmable aberration correction

J Biophotonics. 2014 Jan;7(1-2):29-36. doi: 10.1002/jbio.201300041. Epub 2013 Jun 21.

Abstract

We present a stimulated emission depletion (STED) microscope that provides 3-D super resolution by simultaneous depletion using beams with both a helical phase profile for enhanced lateral resolution and an annular phase profile to enhance axial resolution. The 3-D depletion point spread function is realised using a single spatial light modulator that can also be programmed to compensate for aberrations in the microscope and the sample. We apply it to demonstrate the first 3-D super-resolved imaging of an immunological synapse between a Natural Killer cell and its target cell.

Keywords: STED microscopy; confocal microscopy; fluorescence microscopy; immune synapse; nanoscopy; super-resolution.

Publication types

  • Editorial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Artifacts
  • Cell Line
  • Image Processing, Computer-Assisted / methods*
  • Imaging, Three-Dimensional / methods*
  • Killer Cells, Natural / cytology
  • Microscopy / methods*
  • Microspheres
  • Nanodiamonds / chemistry

Substances

  • Nanodiamonds