[Authentication of Lonicera japonica using bidirectional PCR amplification of specific alleles]

Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(24):3752-7.
[Article in Chinese]

Abstract

Objective: To identify SNP in flos Lonicerae, and authenticate Lonicera japonica from its adulterants and the mixture by using bidirectional PCR amplification of specific alleles (Bi-PASA).

Method: SNP of L. japonica and its adulterants was identified by using ClustulW to align trnL-trnF sequences of the Lonicera genus from GenBank database. Bi-PASA primer was designed and the PCR reaction systems including annealing temperature optimized. Optimized result was performed in 84 samples to authenticate L. japonica, its adulterants and the mixture.

Result: When the annealing temperature was 61 degrees C, DNA from L. japonica would be amplified 468 bp whereas PCR products from all of the 9 adulterants were 324 bp. The established method also can detect 5% of intentional adulteration DNA into L. japonica.

Conclusion: The Bi-SPASA could authenticate L. japonica from its adulterants and the mixture.

Publication types

  • English Abstract

MeSH terms

  • Alleles
  • DNA Barcoding, Taxonomic / methods
  • DNA, Plant / analysis
  • DNA, Plant / genetics*
  • Flowers / genetics
  • Lonicera / classification
  • Lonicera / genetics*
  • Plants, Medicinal / classification
  • Plants, Medicinal / genetics*
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Single Nucleotide*
  • RNA, Transfer, Leu / genetics
  • RNA, Transfer, Phe / genetics
  • Reproducibility of Results
  • Species Specificity

Substances

  • DNA, Plant
  • RNA, Transfer, Leu
  • RNA, Transfer, Phe