[Preparation of agar-paraffin double-embedded longitudinal sections of Schistosoma japonicum]

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2012 Oct 30;30(5):415-7.
[Article in Chinese]

Abstract

Schistosoma japonicum adults are pre-embedded in a double-layer agar and made the block, then dehydrated with alcohol, isobutyl alcohol and n-butyl alcohol. Various staining procedures can be conducted after conventional sectioning and dewaxing. Complete longitudinal serial sections of the pre-embedded worms can be obtained, and the desired sections can be easily located accurately.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Paraffin Embedding / methods*
  • Schistosoma japonicum / anatomy & histology*
  • Staining and Labeling