Endogenous stromal cell-derived factor-1 (CXCL12) supports autonomous growth of acute myeloid leukemia cells

Leuk Res. 2013 May;37(5):566-72. doi: 10.1016/j.leukres.2013.01.016. Epub 2013 Mar 6.

Abstract

We investigated the role of endogenous stromal cell-derived factor-1 (SDF-1; CXCL12) in the survival and proliferation of acute myeloid leukemia (AML) cells in vitro. CD34(+) cells from the peripheral blood of five patients with AML, as well as five AML cell lines, produced and secreted SDF-1. Knock-down of endogenous SDF-1 expression using siRNA technology downregulated the constitutive phosphorylation of SDF-1-related signaling molecules and significantly inhibited spontaneous proliferation of the AML cell lines during a 3-day incubation in serum-free conditions. These results indicate that endogenous SDF-1 expression by AML cells plays a role in the autonomous growth of the cells.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Proliferation*
  • Chemokine CXCL12 / biosynthesis*
  • Chemokine CXCL12 / genetics
  • Female
  • Gene Expression Regulation, Leukemic*
  • Gene Knockdown Techniques
  • HL-60 Cells
  • Humans
  • K562 Cells
  • Leukemia, Myeloid, Acute / metabolism*
  • Leukemia, Myeloid, Acute / pathology
  • Male
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Proteins / genetics
  • Phosphorylation / genetics
  • Signal Transduction*
  • Tumor Cells, Cultured
  • U937 Cells

Substances

  • CXCL12 protein, human
  • Chemokine CXCL12
  • Neoplasm Proteins