Construction and comparative analyses of highly dense linkage maps of two sweet cherry intra-specific progenies of commercial cultivars

PLoS One. 2013;8(1):e54743. doi: 10.1371/journal.pone.0054743. Epub 2013 Jan 31.

Abstract

Despite the agronomical importance and high synteny with other Prunus species, breeding improvements for cherry have been slow compared to other temperate fruits, such as apple or peach. However, the recent release of the peach genome v1.0 by the International Peach Genome Initiative and the sequencing of cherry accessions to identify Single Nucleotide Polymorphisms (SNPs) provide an excellent basis for the advancement of cherry genetic and genomic studies. The availability of dense genetic linkage maps in phenotyped segregating progenies would be a valuable tool for breeders and geneticists. Using two sweet cherry (Prunus avium L.) intra-specific progenies derived from crosses between 'Black Tartarian' × 'Kordia' (BT×K) and 'Regina' × 'Lapins'(R×L), high-density genetic maps of the four parental lines and the two segregating populations were constructed. For BT×K and R×L, 89 and 121 F(1) plants were used for linkage mapping, respectively. A total of 5,696 SNP markers were tested in each progeny. As a result of these analyses, 723 and 687 markers were mapped into eight linkage groups (LGs) in BT×K and R×L, respectively. The resulting maps spanned 752.9 and 639.9 cM with an average distance of 1.1 and 0.9 cM between adjacent markers in BT×K and R×L, respectively. The maps displayed high synteny and co-linearity between each other, with the Prunus bin map, and with the peach genome v1.0 for all eight LGs (LG1-LG8). These maps provide a useful tool for investigating traits of interest in sweet cherry and represent a qualitative advance in the understanding of the cherry genome and its synteny with other members of the Rosaceae family.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles
  • Chromosome Mapping*
  • Gene Frequency
  • Genetic Linkage*
  • Genetic Markers
  • Genotype
  • Phenotype
  • Polymorphism, Single Nucleotide
  • Prunus / genetics*
  • Prunus / growth & development

Substances

  • Genetic Markers

Grants and funding

This work was funded by 07CN13PBT-167 INNOVA Chile CORFO project, and by INRA’s Department of “Genetic and Plant Breeding” and the Conseil Régional d’Aquitaine 2011 project “convention 2011-12 01 00122000568” for analyses performed in France. Additional funding was provided by Universidad Andres Bello DI-78-12/I project, the USDA’s National Institute of Food and Agriculture – Specialty Crop Research Initiative project “RosBREED: Enabling marker-assisted breeding in Rosaceae” (2009-51181-05808), CONICYT Fondecyt/regular number 1120261 and CONICYT Fondecyt/regular number 1121021. CK holds a CONICYT doctoral fellowship (21120115 and 24121484). JC is holder of a postdoctoral fellowship from the “Programa de Formación Postdoctoral de Personal Investigador” of the “Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia”, Spain. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.